Action pattern of Valencia orange PME de-esterification of high methoxyl pectin and characterization of modified pectins.
Identifieur interne : 002755 ( Main/Exploration ); précédent : 002754; suivant : 002756Action pattern of Valencia orange PME de-esterification of high methoxyl pectin and characterization of modified pectins.
Auteurs : Yookyung Kim [États-Unis] ; Quincy Teng ; Louise WickerSource :
- Carbohydrate research [ 0008-6215 ] ; 2005.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Pectins.
- chemical , metabolism : Carboxylic Ester Hydrolases, Pectins.
- enzymology : Citrus sinensis.
- Hydrogen-Ion Concentration, Kinetics, Magnetic Resonance Spectroscopy, Molecular Weight, Potentiometry, Scattering, Radiation.
Abstract
Valencia pectinmethylesterase (PME) fractions, B-PME, containing 36 and 13 kDa protein bands and U-PME, containing a 36 and 27 kDa protein bands, were used to de-esterify original pectin (O-Pec) from 73% degree of esterification (%DE) to 63% (B-Pec) and 61% DE (U-Pec), respectively. Most O-Pec eluted from ion exchange chromatography at low salt concentration and a smaller component eluted at higher ionic strength. B-Pec and U-Pec eluted as one broad peak at higher ionic strength. PME modification did not change molecular weight: O-pectin (134,000 g/mol), U-Pec (133,850 g/mol), and B-Pec (132,250 g/mol). The NMR signal of GG and GGG increased after modification, whereas the signal of EE and EEE decreased. The negative zeta-potential increased with pH for all pectins. U-PME and B-PME created differently modified pectins that vary in degree and length of multiple attacks and fraction of the pectin population that was modified.
DOI: 10.1016/j.carres.2005.09.013
PubMed: 16216228
Affiliations:
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Le document en format XML
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Most O-Pec eluted from ion exchange chromatography at low salt concentration and a smaller component eluted at higher ionic strength. B-Pec and U-Pec eluted as one broad peak at higher ionic strength. PME modification did not change molecular weight: O-pectin (134,000 g/mol), U-Pec (133,850 g/mol), and B-Pec (132,250 g/mol). The NMR signal of GG and GGG increased after modification, whereas the signal of EE and EEE decreased. The negative zeta-potential increased with pH for all pectins. U-PME and B-PME created differently modified pectins that vary in degree and length of multiple attacks and fraction of the pectin population that was modified.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><noCountry><name sortKey="Teng, Quincy" sort="Teng, Quincy" uniqKey="Teng Q" first="Quincy" last="Teng">Quincy Teng</name><name sortKey="Wicker, Louise" sort="Wicker, Louise" uniqKey="Wicker L" first="Louise" last="Wicker">Louise Wicker</name></noCountry><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Kim, Yookyung" sort="Kim, Yookyung" uniqKey="Kim Y" first="Yookyung" last="Kim">Yookyung Kim</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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